Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)

Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae) Presentations

American Journal of Botany

• Author(s): Waycott, M., Jones, BL., Van Dijk, JK., Robson, HLA., Calladine, A.
• Published: 2011
• Volume: 98

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Abstract: • Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. • Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. • Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.

• Urls: https://bsapubs.onlinelibrary.wiley.com/doi/10.3732/ajb.1000356
• Publisher Location: Alice Springs